Figure 4.

Growth characteristics of pocket-protein-deficient MEFs. (A) Growth curves of wt, Rb^−/−, Rb^−/−p107^−/−, and TKO MEFs at passages 3 (squares), 5 (circles), and 7 (triangles). The figure shows a representative of three independent experiments, each performed in triplicate. (B) Cell proliferation on a 3T9-based protocol of indicated MEFs for 20 passages. At 3.5-d intervals, the total numbers of cells of three independent cultures of each genotype were determined before redilution of the cells to 3 × 10⁵ per six-well plate (input). The experiment was performed twice. Error bars indicate standard deviations of the mean.