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Immortal TKO MEFs do not grow anchorage independently
| Genotype MEFs
|
Exp I
|
Exp II
|
Exp III
|
|---|---|---|---|
| wt | 0 | 0 | 0 |
| Rb−/− | 0 | 0 | 0 |
| RB−/−p107−/− | 0 | 0 | 0 |
| Rb−/−p107−/−p130−/− | 0a | 0 | 0 |
| p53−/− + RASV12 | 229 | 238 | 273 |
Number of foci of indicated early passage MEFs appearing after 2 wk in soft agar.
A small percentage of the MEFs developed into tiny colonies (up to 20 cells) but did not sustain growth.
