Figure 1.

VAMP7 Colocalizes with Atg16L1 and Atg5

(A) Colocalization between LC3-CFP, Atg16L1-GFP, Atg16L1-Flag, Atg5-GFP, VAMP7-mRFP, endogenous VAMP7, and stably expressed VAMP7-HA. Cells were transfected for 20 hr. Confocal colocalization is indicated. At least 20 cells were analyzed in each condition. Error bars = standard deviation (SD). Scale bar, 5 μm.

(B) HeLa cells stably expressing VAMP7-HA were transfected for 20 hr with Atg16L1-GFP. The cells were processed for double immunogold labeling with anti-HA antibodies (15 nm gold particles) and anti-GFP antibodies (10 nm gold particles) for electron microscopy. Colocalization between Atg16L1 structures with VAMP7-HA gold particles is shown. n = 17 cells. Scale bar, 500 nm. Error bars = standard error of the mean (SEM).

See also Figure S1.