Figure 3.

Novel object test for anxiety-related behavior in wt male, Tfm male, and wt female rats. (A) The amount of time spent with and number of visits to the novel object. (B) The number of grid crossing/rearings and (C) time spent grooming in the open field test. Tfm males showed increased indices of anxiety, exhibiting decreased time spent with the novel object compared to both wt males and females, and fewer visits to the object compared to wt females (A). The number of grid crossings and rearings both suggest that motor activity was comparable between wt and Tfm males, although reduced compared to wt females. No significant differences were found for time spent grooming. Test duration was 300 seconds. * indicates p<0.05, ** p<0.01.