Fig. 5.

Increased Sus cells in Gdf11^–/– and ActβB^–/–;Gdf11^–/– OE. (A) ISH with the indicated probes indicates increased Sox2 expression and a thicker Sus layer (white arrowhead) in ActβB^–/–;Gdf11^–/– OE. (B) Magnified images show apical SOX2⁺ cells outlined by white boxes. (C) Most apical SOX2⁺ cells are also SUS4⁺ [83±2% (±s.e.m.)]. (D) Mean number of SOX2⁺ Sus cells/mm OE (± s.e.m.; ^*, P≤0.05 DT). (E) SOX2⁺ Sus cells and the percentage of these that are BrdU⁺ (`proliferation index') plotted as mean ± s.e.m. dKO, ActβB^–/–;Gdf11^–/–. BL, basal lamina; N, neuronal layer; Sus, sustentacular layer. Scale bars: 20 μm in A,B; 10 μm in C.