Fig. 2.

The frv locus encodes the transmembrane protein Tmem2. (A) Meiotic and physical maps of the zebrafish genomic region containing frv. Meiotic mapping revealed a 0.28 cM interval for frv defined by SNPs on the ends of overlapping contigs (BX323080, BX294191). Fractions indicate the number of proximal (red) and distal (blue) recombination events out of 2528 meioses. Five candidate genes are shown as represented in Ensembl. (B) The predicted structures of Tmem2 protein in wild-type and frv mutant embryos. Amino acid positions defining the domains are indicated. Histograms demonstrate that frv mutants contain a C-to-T transition at position 2623 of the tmem2 coding region. This mutation changes an Arg codon to a premature stop codon at amino acid position 875 and is predicted to result in a truncated Tmem2 protein.