Fig. 3.

Fez is not involved in the differentiation of serotonergic neurons. (A) Experimental flow to investigate whether Fez functions in neural differentiation. Orange and green show the intact egg cytoplasm and the reagent that we injected, respectively. (B-G′) Serotonergic neurons and synaptotagminB (synB)-positive neurons in pluteus larvae subjected to the following treatments: glycerol alone (B), DAPT (C), Fez morpholino (D), DAPT and Fez morpholino (E), Fez mRNA injection (F), and DAPT and Fez mRNA injection (G). The anterior regions of embryos indicated by the boxed area in the middle panels are magnified in the right-hand panels. A double arrow shows the direction of oral (o)-aboral (ab) axis. Scale bar: 20 γm.