Fig. 1.
Ngn2 undergoes cell cycle-regulated phosphorylation. (A) In vitro translated (IVT) [35S]methionine-labelled xNgn2 and 9S-A xNgn2 were incubated in interphase (I) and mitotic (M) Xenopus egg extracts before SDS-PAGE and autoradiography. In M extracts, phosphorylated xNgn2 runs at the level of the 36 kDa molecular weight marker (indicated). (B) Western blot analysis of HA-tagged mNgn2 and mutants thereof, transfected into mouse P19 cells with and without λ-phosphatase treatment. (C) Western blot analysis of myc-mNgn2 from E16.5 mouse cortex, with and without λ-phosphatase treatment.