Figure 4.

del-1::GFP and acr-5::GFP are expressed ectopically in A-type motor neurons of unc-4 and unc-37 mutants. GFP expression (green) was visualized in mid- to late L2 larval animals. Autofluorescent gut granules are yellow. (A–F) The del-1::gfp reporter gene is regulated by 1.8 kb of del-1 upstream sequence. In the ventral nerve cord of wild-type L2 animals, del-1::GFP is expressed in the VB motor neurons but is prematurely expressed in the VAs during this period in unc-37(e262) and in unc-4 mutants. (G–L) The acr-5::gfp reporter gene is regulated by 4.2 kb of acr-5::gfp upstream sequence. acr-5::GFP is expressed in B-type motor neurons (DB, VB) in ventral nerve cords of wild-type animals. In unc-4 and unc-37 mutants, acr-5::GFP is ectopically expressed in A-type motor neurons (DA, VA). Genotypes for each row are indicated at left. unc-37(e262) corresponds to UNC-37(H539Y); unc-4(e2321) corresponds to UNC-4(R197K). (C,I) Enlargements of areas indicated by insets in B and H, respectively. Unlabeled motor neurons in E, F, K, and L are identified at top. Bars, 20 μm. Bar in B applies to B and H; bar in C applies to C–L.