Figure 6.

UNC-4 and UNC-37 interact in yeast two-hybrid assays. UNC-4 proteins are fused to the GAL4 DNA-binding domain; UNC-37 proteins are fused to the GAL4 activation domain. Assays for growth on His⁻ plates were performed in the presence or absence of 3-AT: (+) growth on 100 mm 3-AT; (−) weak or no growth on 100 mm 3-AT. (A) Interactions between full-length UNC-37 and UNC-4 deletion mutants. UNC-4 refers to the full-length protein; UNC-4C represents the carboxy-terminal truncated protein (152–252). Other UNC-4 truncations are denoted by amino acid boundaries. (B) Interactions of UNC-37 and UNC-37(E580K) with UNC-4 eh1 region missense mutations. The allele numbers of previously identified unc-4 mutations are indicated. Asterisks indicate relative positions of mutations. (C) Selected growth assays ± 100 mm 3-AT from A and B.