Table 2.
UNC-4/UNC-37-dependent repression of del-1::GFP and acr-5::GFP
| Genotype
|
del-1::GFP
|
acr-5::GFP
|
||||
|---|---|---|---|---|---|---|
| %VAs
|
%VBs
|
%DAs
|
%DBs
|
%VAs
|
%VBs
|
|
| Wild type | 17 | 100 | 5 | 100 | 1 | 95 |
| unc-37(e262) (H539Y) | 98 | 100 | 94 | 97 | 76 | 84 |
| unc-4(wd1) | 96 | 100 | 94 | 100 | 79 | 94 |
| unc-4(e521) (R197K) | 100 | 100 | 98 | 99 | 85 | 89 |
| unc-4(e2307) (G188S) | 98 | 100 | 96 | 99 | 83 | 89 |
| unc-4(e2323) (R190Q) | 96 | 100 | 93 | 100 | 49 | 94 |
| unc-4(e2322ts) (L121F) | 94 | 100 | 63 | 94 | 54 | 81 |
| unc-4(e120) | 97 | 100 | 98 | 97 | 82 | 87 |
| unc-37(d);aunc-4(e120) | 96 | 100 | 98 | 99 | 70 | 84 |
| unc-4(e26) (G188D) | 95 | 99 | 98 | 96 | 77 | 92 |
| unc-37(d);unc-4(e26) | 98 | 100 | 99 | 100 | 84 | 95 |
| unc-4(e2321) (R197K) | 95 | 100 | 95 | 99 | 82 | 89 |
| unc-37(d);unc-4(e2321) | 50 | 100 | 88 | 97 | 35 | 85 |
| unc-37(d) | 3 | 100 | 0 | 97 | 0 | 87 |
Larval (L2) animals (n ≥ 15) were scored for each genotype. Neurons scored per animal: 9–10 VAs; 9 VBs, 6 DAs; 5 DBs. unc-4 and unc-37 genotypes are shown at left; allele names and amino acid substitutions are indicated in parentheses. Percentage of GFP-positive neurons for each genotype are listed in the columns for each motor neuron class.
a
unc-37(d) corresponds to the UNC-37(E580K) mutation.