Skip to main content
. 1998 Sep 1;12(17):2698–2710. doi: 10.1101/gad.12.17.2698

Figure 3.

Figure 3

Histone HI kinase activity associated with Clb5 during sporulation is Cdc28 dependent. (A) Whole cell extract (50 μg) or anti-HA immunoprecipitates (from 1 mg of total protein) from either wild-type diploid cells (No Tag) or CLB5HA/CLB5HA diploids were separated by gel electrophoresis and probed for Clb5HA and Cdc28. (B) Histone H1 kinase activity associated with Clb5HA immune complexes prepared from diploid strains expressing wild-type Cdc28 (CLB5HA CDC28), a temperature-sensitive Cdc28-4 (CLB5HA cdc28-4), or a wild-type Cdc28 with untagged CLB5 (No Tag). Strains were grown and induced to sporulate at the permissive temperature of 28°C and kinase activity was assayed at 25°C. (C) DNA content over a time course of sporulation of wild-type diploids and diploids that express a stabilized version of the Clb/Cdc28 CDK inhibitor SIC1 (SIC1ΔP) under the regulation of the meiosis specific IME2 promoter.