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. 2011 Aug 19;10:244. doi: 10.1186/1475-2875-10-244

Figure 1.

Figure 1

Optimization of reagents for real-time PCR. Amplification curves (left panels) and melt curves (right panels) from Plasmodium falciparum gDNA spiked into uninfected blood and tested by real-time PCR. SYBR® Green dye concentrations of 10× (A), 20× (B), and 40× (C) were tested with 5% (red) and 10% (blue) volumes of blood in the PCR reaction. Uninfected blood serves as a negative control (gray). The stippled line marks the threshold for the amplification curves. Samples were run in triplicate.