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Template dilution in blood. Genomic DNA from a patient sample with Plasmodium falciparum was serially diluted ten-fold in water and spiked into whole blood for direct PCR. Amplification curves (A) and melt curves (B) of reactions performed in triplicate using the Plasmodium screening primers. Uninfected blood served as the negative control (gray). The stippled line marks the threshold for the amplification curves.
