Figure 1.

Pdd1p and Pdd2p are detected in parental macronuclei as well as anlagen during conjugation. Total proteins from micro- and macronclei isolated from 6-hr conjugants and from micronuclei and developing new macronuclei from conjugating cells at 14 hr postmixing were resolved on 10% SDS–polyacrylamide gel, transferred to membrane, and probed with Pdd2p peptide-derived antibodies. Blots were stripped and reprobed with Pdd1p- and, as a loading control, histone H2B-specific antibodies. Asterisks denote slow-migrating isoforms of Pdd2p (see Madireddi et al. 1996; Smothers et al. 1997b).