Figure 2.

Nuclear localization of Pdd2p during conjugation. Selected stages of conjugation are shown schematically and by micrographs of wild-type Tetrahymena fixed at indicated time points and stained by DAPI and Pdd2p antibodies. (A) Tetrahymena development before formation of anlagen; (B) late conjugation stages after anlagen formation. (Mi) Micronuclei; (An) anlagen; (Old Ma) old macronuclei (●). Several intranuclear structures, associated with DNA elimination (doughnut-like structures) are indicated by white arrowheads.