Table 2.
Plasmids expressing RNase E polypeptides
| Vector (restriction sites)
|
rne insert
|
Plasmid (residues encoded)
|
|---|---|---|
| pET11a (NdeI–EcoRI) | NdeI–ApoI fragment | pET–rne (1–1061) |
| pET11a (NdeI–BamHI) | NdeI–BstYI from pET–rne | pET–rneA (1–263) |
| pET11c (BamHI) | BstYI–BamHI from pET–rne | pET–rneB (263–844) |
| pET11a (NdeI–BamHI) | NdeI–BamHI from pET–rne | pET–rneC (1–844) |
| pET11b (BamHI–EcoRI) | BamHI–ApoI from pGEM–rneD | pET–rneD (185–1061) |
| pET11c (BamHI filled) | PCR-generated fragmenta | pET–rneHC1 (501–843) |
| pET11c (BamHI filled) | PCR-generated fragmentb | pET–rneHC2 (628–843) |
a
Primers rne2041 and rne3069.
b
Primers rne2422 and rne3069.