Table 3.
Primers used in this study
| Name
|
Sequence (from 5′ to 3′)a
|
|---|---|
| rne215 | ggatccTACGGAATAACCCATTTTGCCCGAC |
| rne541 | atATGAAAAGAATGTTAATCAACGCAACTCAGC |
| rne627 | GATATCCAGGTCcTACAGACGCTGCCC |
| rne1192 | ATGCGCGAACGATTACGTTGCTCT |
| rne2041 | ATGCTGCCGAAGCTGCATGAAGAA |
| rne2064 | TTCTTCATGCAGCTTCGGCAGCATG |
| rne2422 | GAACGTACTGAAGGCAGCGATAATC |
| rne2445 | ATTATCGCTGCCTTCAGTACGTTC |
| rne2721 | CTGATTGAGCTGACGCTGTTTACGACGCGGC |
| rne2754 | TTCTTCGGCTACGCTTTGCTCGTAACGC |
| rne3069 | CCAGACTTTGCCAGAGGCCAGTTC |
| rne3076 | TATCCAATTGTACGTCCGCAAGATGTAC |
| rne3676 | GCAACACGTCATGCCTCTGCCG |
| rneYSL1 | GAGTAAgaattcATGAAAAGAATGTTAATC |
| rneYSL2 | GCGCGctcgagTTACGCAGGTTGTTCCGGACGCTTAC |
| rneYSL3 | CCAACCgaattcTACATGCTGCCGAAGCTG |
| rneYSL4 | TTCCTGctcgagTTATTCGGCAGCGACAGTTTC |
| rneYSL5 | CGTTACgaattcAGCGTAGCCGAAGAAGCG |
| rneYSL6 | ACTTTGctcgagTTATTAtTCcACAGGTTG |
| pnp1 | atATGCTTAATCCGATCGTTCGTAAATTCC |
| pnp2 | TTACTCGCCCTGTTCAGCAGCC |
| pnpYSL1 | GGGCGgaattcATGCTTAATCCGATCGTT |
| pnpYSL2 | GGGGGctcgagTTACTCGCCCTGTTCAGC |
| rhlB607K | ATTCAGGTGGTGGTACTGGACaAAGCCGATCGCA |
| rhlB1282R | ATTTGCTTACCGGAATTGAGT |
| rhlBYSL1 | GGGCGgaattcATGAGCAAAACACATTTAACAG |
| rhlBYSL2 | GGGGGctcgagTTAACCTGAACGACGACGATTACGCGG |
| enoYSL1 | CTTGAGGggatccTAATGTCCAAAATCGTAAAAATC |
| enoYSL2 | CTAGTTctcgagTCAGATAAAGTCAGTCTTATG |
| enoYSL3 | CTTGAGGAActcgagATGTCCAAAATCGTAAAAATC |
| enoYSL4 | GCTAGTTctcgagTCAGATAAAGTCAGTCTTATG |
a
The lowercase letters, showing the substitutions introduced to manufacture restriction sites, prevent secondary structure formation within the primer or create mutations.