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. 2011 Sep 12;6(9):e24307. doi: 10.1371/journal.pone.0024307

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Orecchia et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Sirt1 was stained with the primary antibody (left panels), and endothelial cells were put in evidence with a co-staining for the PECAM/CD31 transmembrane adhesion molecule (middle panels). Merged pictures are in the right panels. Each panel is a total projection of a confocal stack of images; bars = 20 µm. (B) HDMEC were treated with the indicated cytokines and the cell lysates were analyzed by immunoblotting with an anti-Sirt1 antibody. An antibody against tubulin was used as a loading control. A representative experiment is reported and the densitometric analysis of at least three different experiments is shown as the mean ± s.d.