Figure 3.

Schematic reconstruction of a signalling pathway through synthetic lethality data. (a) A situation in which there are two pairs of complementary pathways ( and ). (b) Model of the situation as follows: the primary knockouts are considered signals (they are not observed). As those are our genes of interest, they will also form the core nodes. The secondary effects are accessible to observation and, therefore, represented by the effects , and . Each SL pair is connected by a dashed line. (c) NEMs that might be estimated from (b), using binary observables and one of the approaches in Sections 5.1 or 5.2.