Figure 3. miRNA expression patterns 1 hour after chemical stimulation with NMDA and bicuculline to 15 days cultured rat embryonic hippocampal neurons.

(A) Relative ARC transcript levels are increased after stimulation with NMDA or bicuculline. (*p<0.05, ***p<0.001, t-test, mean ± S.E.M.). (B) Mean relative fold changes compared to unstimulated neurons were computed for each stimulation method. The amplitude of expression changes was remarkably correlated. (rS = 0.81, p = 7.10⁻⁴¹). (C) miRNAs with expression significantly different from the one in unstimulated neurons. (p<0.05, Kruskal-Wallis test). (D) 32 miRNAs showed expression changes in common after NMDA and bicuculline stimulation. (unstimulated; n = 10, NMDA; n = 10, 60 µM, 5 min treatment, bicuculline; n = 8, 20 µM, 1 hour treatment).