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. 2011 Sep 12;6(9):e24682. doi: 10.1371/journal.pone.0024682

Figure 6. Contextual conditioning affects transcript levels of miRNA biogenesis enzymes and RISC components.

Figure 6

(A) Transcript levels of DGCR8, Drosha, Dicer1, Ago1, Mov10 and GW182 in vivo hippocampal CA1 region after contextual fear conditioning. (*p<0.05, ANOVA, home cage: n = 12, 1 h and 3 h: n = 12, 24 h: n = 8. normalized by GAPDH, mean ± S.E.M.) FC =  fear conditioning. (B) Transcript levels of DGCR8, Drosha, Dicer1, Ago1, Mov10 and GW182 in in vitro cultured neurons after stimulation with NMDA or bicuculline. (*p<0.05, t-test, n = 8 per group from four different biological samples, normalized by GAPDH, mean ± S.E.M.) (C) Protein levels of DGCR8 and Drosha after neuronal activation. (*p<0.05, t-test, n = 3 from three different biological samples, normalized by GAPDH, mean ± S.E.M.).