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. 2011 Sep 12;6(9):e24584. doi: 10.1371/journal.pone.0024584

Figure 2. Opposite effects of MiR-34a on Notch1 and Notch2 receptors throw the direct targeting of Dll1 in the MB Daoy cell line.

Figure 2

A. Normalized cell index (means ±SD) as a measure for proliferation of Daoy cells treated with SNALPs carrying miR-34a or with SNALP-scrambled. Treatment was initiated 20 h post seeding. B. Representative Western blot for Daoy WT and Daoy stable Dll1 clone #1. The cells were plated at high density (H) or low density (L), as illustrated. Anti-NICD2 and anti-β-Actin antibodies were used. C. Representative Western blot for Daoy cells plated at different densities and transfected with wild-type or seed-mutated miR-34a, and infected with adenovirus carring AdV-GFP-miR-34a and AdV-GFP-mock using anti-NICD1, anti-NICD2 and anti-β-Actin antibodies. D. Representative Western blot for Daoy stable Dll1 clone #1 cells plated at different densities and transfected with wild-type or seed-mutated miR-34a, using, anti-NICD2 and anti-β-Actin antibodies. E. Representative Western blot analysis for Daoy Dll1 clone #1 cells plated at different densities, under basal conditions or at 14 h from transfection with miR-34a or with an empty vector, using anti-Hes1 and anti-β-Actin antibodies.