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. 2011 Sep 12;6(9):e24584. doi: 10.1371/journal.pone.0024584

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de Antonellis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Real-time PCR showed the time-dependent expression of miR-34a following tetracycline single-pulse stimulation. Data are means ±standard deviation of 3 experiments, each carried out in triplicate. B. Top: Representative Western blot for time-course of tetracycline-stimulated Daoy-TR-EV and Daoy-TR-miR-34a cells, using an antibody panel against: Dll1, CyclinD1, cMYC, CDK4 and β-Actin. C. Densiometric time-course analyses (Dll1, cMYC, CDK4), as β-Actin normalized, each value was expressed as fold-stimulation over the unstimulated cells (t0). Data are means ±standard deviation of 3 experiments, each carried out in triplicate.