Figure 2.

Electrophoretic analysis of RNAs synthesized from promoters containing mutations at the inverted repeats. In vitro transcription assays were carried out on wild-type or mutant promoter-containing DNAs at physiological superhelical density in the presence of Eco SSB at a 1:1 Eco SSB/DNA ratio (wt/wt) . The sequence from −18 to +1 of the template strand of the promoters is presented above the results of the transcription assays. In each case, sequence changes from promoter P1 are presented in boldface type. The location of the inverted repeats is indicated by arrows above the sequence.