Table 3. The effect of pertuzumab on EGFR dimerization in A431 and SKOV3 cells.

A431
	Without EGF			With EGF
Effect of:	Complete medium	Gefitinib+complete medium	Gefitinib +serum free medium	Complete medium	Gefitinib+complete medium	Gefitinib +serum free medium
Pertuzumab	0.9±0.2	1.1±0.3	1.5±0.1	1.0±0.0	1.5±0.1	1.1±0.1

The table presents EffectValues, i.e. quotients of dimerization degrees. Lysates within the same gel treated identically apart from the exposure of pertuzumab were used as references. Data are presented as mean±S.E (n = 4).

*indicates treatments affecting dimerization, i.e. with a calculated effect significantly different from 1 (p<0.1). Pertuzumab disrupts approximately 50% of the dimerization in SKOV3 cells, regardless of treatment of EGF, FCS or gefitinib. No effects of pertuzumab on EGFR dimerization in A431 cells are observed.