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. 2011 Sep 1;17:2368–2379.

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Copyright © 2011 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Real-time RT–PCR of β subunits from whole LG (left panels) and epithelial cells collected by LCM (right panels). mRNA levels of β1 and β3 from whole LG of IAD rabbits were significantly lower than control animals (p<0.05), whereas no significant difference was detected between control and IAD animals for β2 (p>0.05). β1: In epithelial cells collected by LCM, β1 was most abundant in acini, and their level in IAD appeared to be similar in duct segments and was significantly lower than control animals [13] in acini and all duct segments (p<0.05). β2: mRNA was least abundant in acini and most abundant in interlobar duct, and its levels in IAD rabbits were significantly lower than control animals in acini and all duct segments except in intralobar duct (p<0.05). β3: mRNA was least abundant in acini while its levels were similar in all duct segments. Compared to control animals [13], its levels in IAD animals were significantly lower in acini (p<0.05), while no differences were detected in duct segments (p>0.05). D4: intralobular duct. D3: interlobular duct. D2: intralobar duct. D1: interlobar duct. Data were presented as mean±SEM.