Fig. 4A–H.

Representative constructs cocultured in the (A–D) absence and (E–H) presence of a feeder cell monolayer at Day 28 of culture stained with (A, E) alcian blue, (B, F) picrosirius red, (C, G) collagen I, and (D, H) collagen II are shown (scale bar = 200 μm). Cocultured constructs produced more extensive and organized tissue development, as assessed by staining intensity from histology and immunohistochemistry.