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Experimental study design. Biotinylated insulin-like growth factor-1 (bIGF-1) was bound to a fluorescently labeled streptavidin molecule (strep-488) and adsorbed to the self-assembling peptide scaffold (KLDL)3 (eg, KLD) or tethered through incorporation of biotinylated-KLD (bKLD). In all cases, chondrocytes were encapsulated in the hydrogel.
