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Biotinylated insulin-like growth factor-1 (bIGF-1) is bioactive. Chondrocytes were encapsulated in KLD at 12 × 106 cells/mL and cultured in medium with different soluble factors for 4 days. (A) sGAG and (B) DNA retained after culture in medium with soluble IGF-1 or soluble bIGF-1. (C) sGAG and (D) DNA retained after culture in medium with soluble bIGF-1 or soluble bIGF-1 + soluble streptavidin. Mean ± standard deviation. *Versus no IGF, p < 0.05.
