Table 1.
Soluble and adsorbed transforming growth factor-β1 (TGF-β1), but not tethered, promotes sGAG production. sGAG and DNA content (µg/gel) of BMSCs encapsulated in KLD gels with or without TGF
| Time point | No TGF-β1 (n = 8) | Soluble TGF-β (n = 12) (10 ng/mL) | Adsorbed TGF-β1 (n = 16) (100 ng/mL) | Tethered TGF-β1 (n = 4) (100 ng/mL) | Tethered TGF-β1 (n = 4) (500 ng/mL) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| sGAG | DNA | sGAG | DNA | sGAG | DNA | sGAG | DNA | sGAG | DNA | |
| Day 7 | 6.0 ± 2.2 | 1.8 ± 0.6 | 19.3 ± 10.9* | 2.3 ± 0.9 | 12.3 ± 9.5 | 1.6 ± 0.8 | 3.5 ± 0.7 | 1.2 ± 0.5 | 5.0 ± 0.6 | 1.4 ± 0.2 |
| Day 14 | 6.3 ± 1.9 | 2.0 ± 0.3 | 57.7 ± 33.5 | 3.2 ± 2.6 | 26.5 ± 17.9* | 2.2 ± 0.7 | 4.6 ± 0.7 | 1.9 ± 0.9 | 5.0 ± 1.5 | 1.0 ± 0.4* |
| Day 21 | 7.6 ± 2.2 | 2.3 ± 0.3 | 110.9 ± 49.6* | 4.8 ± 2.6* | 35.1 ± 21.3* | 2.3 ± 0.8 | 5.7 ± 0.5 | 3.1 ± 0.9 | 8.4 ± 1.2 | 1.7 ± 0.7* |
* Versus no TGF-β1, p < 0.05. Mean ± standard deviation.