Figure 4.

Expression of the mea-1 allele during early seed development. Amplification of MEA-1 (top) and ACTIN-11 (ACT11) as a control for cDNA synthesis. RNA was isolated from siliques derived from self-pollinated mea-1 homozygous pistils (mea × mea), self-pollinated wild-type pistils (wt × wt), a cross between a homozygous mea-1 female and wild-type male (mea × wt), and a cross between wild-type female and homozygous mea-1 male (wt × mea). Primers that specifically amplify the mea-1 allele under these PCR conditions were used for RT–PCR. (M) Marker lane; (G) genomic DNA as a control.