Figure 4.

Transcriptional activation in a taf17^ts mutant. DLY983 (TAF17ace1Δ) and DLY984 (taf17^tsace1Δ) strains were transformed with following plasmids: p316:ACE1 (ACE1), pAC–HSF (HSF), pAC–GCN4 (GCN4), and pAC–GAL11 (GAL11). Cell growth, copper induction, and S1 assays of CUP1 RNA were done as in Fig. 1A. The average induced levels in mutant relative to wild type are shown as percentages with standard deviations from three experiments (except two for HSF). (A) Activation domain of Ace1 and HSF, but not Gcn4, can activate Taf17-independent transcription. (B) Activation by artificial recruitment of Gal11 is Taf17 independent.