Table 1.
Variables | Human GeneChip® | Human GeneChip® |
---|---|---|
ST 1.0 | U133 Plus 2.0 | |
Number of probes | 764,885 | >54,000 |
Probe design | 26 probes per gene on average with at least 1 probe per exon | 11 probes are designed to the first 600–800bp of a gene |
Number of transcripts | 19,734 | >47,000 |
Genes well annotated | >28,869 | >38,500 |
Alternative splicing | some data available | not available |
Hybridization cocktail volume | 80 μl | 200 μl |
Sample required | 25 ng/μl | 50 ng/μl |
Cost | lower | higher |
Note. Annotations provided are the most current brochure information. Frequently updated annotation files are available to users on the Affymetrix website. ST denotes sense target.