. Author manuscript; available in PMC: 2012 Oct 1.

Published in final edited form as: Res Nurs Health. 2011 Aug 2;34(5):408–418. doi: 10.1002/nur.20448

Table 2.

RNA Quantity and Quality from Fresh and Frozen Whole Blood Samples

Samples	260/280	RNA Yield (μg)	RIN
Fresh Samples
1A	2.00	14.4	8.9
2A	1.99	3.3*	9.0
Frozen Samples
1B	2.11	9.0	9.0
2B	2.09	8.0	9.0

Note. RNA denotes ribonucleic acid. RIN indicates ribonucleaic acid integrity number. The sample indicated with an asterisk had less whole blood due to less blood vacuuming through the filter due to novice use. This resulted in a smaller yield; however, the RIN number quantity was sufficient for microarray processing. The ratio between light absorbance at 260 and 280 nm (260/280) indicates the quality of RNA.