Immunohistochemical analysis was performed to evaluate the effect of CBL treatment interruption on neurodegeneration in APP tg mice. (a) Levels of the synaptic marker, synaptophysin, in the neocortex of saline-treated non tg and saline or CBL-treated APP tg mice at TPT 0m, TPT 3m and TPT 6m. (b) Levels of synaptophysin, in the hippocampus of saline-treated non tg and saline or CBL-treated APP tg mice at TPT 0m, TPT 3m and TPT 6m. (c) Levels of the neuronal marker, NeuN, in the neocortex of saline-treated non tg and saline or CBL-treated APP tg mice at TPT 0m, TPT 3m and TPT6m. (d) Levels of NeuN, in the hippocampus of saline-treated non tg and saline or CBL-treated APP tg mice at TPT 0m, TPT 3m and TPT 6m. (e) Levels of the astroglial marker, GFAP, in the neocortex of saline-treated non tg and saline or CBL-treated APP tg mice at TPT 0m, TPT 3m and TPT 6m. (f) Levels of GFAP, in the hippocampus of saline-treated non tg and saline or CBL-treated APP tg mice at TPT 0m, TPT 3m and TPT 6m. Error bars represent mean ± SEM. (*)Indicates p<0.05, when comparing APP tg mice with saline-treated non tg mice assessed by one-way ANOVA with post-hoc Dunnett’s and (#) indicates p<0.05 when comparing CBL-treated APP tg mice with saline-treated APP tg mice assessed by one-way ANOVA with post-hoc Dunnett’s.