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. 1999 Nov 15;13(22):2958–2970. doi: 10.1101/gad.13.22.2958

Figure 1.

Figure 1

tam-1 effects on a repetitive myo-3::gfp transgene. An integrated myo-3::gfp transgene was examined in wild-type and tam-1(cc567) mutant backgrounds. (A) Wild-type and (B) tam-1(cc567) animals photographed under fluorescent illumination showing GFP in bodywall muscles (the array produces GFP in both nuclear and mitochondrial compartments). Scale bars, 55 μm. Quantitative measurements of GFP fluorescence in wild-type and tam-1(cc567) mutant backgrounds. Differences in fluorescence level were most striking in the central region of the animal; thus quantitations were carried out in bodywall muscle nuclei in this region. Eight animals were analyzed for each genetic background. Fluorescence was quantitated for six nuclei in each animal with a Nikon U-III multipoint sensor system. Linearity of fluorescence measurements in the range of assay was confirmed by a series of neutral density filters. (wild-type mean= 140.9, wild-type (s.e.m.)= 5.9; tam-1 mean = 7.2, tam-1 s.e.m. = 0.4). A second measure of tam-1 effects on the expression of a repeated transgene is observed with a lin-3 transgene (syIs1). The average induction of vulval precursor cells (VPCs) caused by the expression of syIs1 in wild type is 5.8 VPCs induced (n = 20), however, in tam-1(sy272), there were 3.2 VPCs induced (n = 1 0) (data not shown).