Figure 2.

Assessment of tam-1 effects in a nonrepetitive context. A nonrepetitive transgene array (ccEx6172) was compared in wild-type and tam-1(cc567) mutant backgrounds. (A) Wild-type and tam-1(cc567) animals photographed under fluorescent illumination showing GFP in bodywall muscles (the array produces GFP in nuclei). Scale bar, 55 μm. (B) Quantitation of fluorescence was carried out in six nuclei in the central region of the body for each of eight animals as in Fig. 1B. (wild-type mean = 15.2, wild-type s.e.m. = 1.4; tam-1 mean = 15.6, tam-1 s.e.m. = 1.7). (C) Analysis of repetitive (simple) and nonrepetitive (complex) transgene activity in wild-type, tam-1(cc567) and lin-35(n745) backgrounds. Transgenes are as described in Materials and Methods.