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. Author manuscript; available in PMC: 2011 Sep 13.
Published in final edited form as: Exp Cell Res. 2009 May 12;315(14):2420–2432. doi: 10.1016/j.yexcr.2009.05.004

Figure 5.

Figure 5

Analysis of apoptotic response and survival of 293T, 293T/hMSH5, and 293T/hMSH5P29S cells following IR exposure. (A) TUNEL analysis of IR triggered apoptotic responses. Cells were irradiated with 2, 5, and 10 Gy IR, and the levels of apoptosis were analyzed 24 h later. Without IR treatment and under identical experimental conditions, approximately 1% of cells were detected to be apoptotic for all three types of cells. Imatinib was used to inactivate c-Abl. The mean percentages and standard deviations (error bars) were determined from three independent data points. Statistically significant differences between cell lines are denoted with an asterisk. (B) Clonogenic survival analysis of 293T, 293T/hMSH5 and 293T/hMSH5P29S cells treated with IR. Colonies that contained at least 50 cells were counted and the percentage of cell survival was determined in reference to untreated control cells. The means of three individual experiments and standard deviations (error bars) are presented, and asterisks are used to indicate significant differences between data points. (C) Analysis of cas-pase-3 activation in 293T, 293T/hMSH5, and 293T/hMSH5P29S cells. Cells were treated with 10 Gy IR and analyzed after an additional 24 h in culture. Immunoblotting was conducted by the use of an α-active caspase-3 antibody. Imatinib was used to inhibit c-Abl, and α-tubulin blot was utilized as a loading control. kDa, molecular weight (Mr) in thousands.