Figure 3.
Effect of exercise on neurotrophin-3 (NT-3) messenger RNA (mRNA) and protein synthesis. Moderately intense exercise training did not change NT-3 mRNA expression in the soleus or gastrocnemius muscle (A and B, P>.05). In contrast, the effect of exercise training did increase NT-3 protein levels (C and D). The increased NT-3 protein levels were not significant in the soleus muscle (C, P>.05) but were significant in the gastrocnemius muscle (D, P<.05). The NT-3 mRNA data (A and B) are represented in threshold cycle (CT) value, where lower values indicate a higher amount of mRNA in the sample. In addition, comparisons between groups (B) are indicated by fold change (2ΔCT-ΔCT). Data represented as mean scores; error bars indicate standard error of the mean. Group comparisons with P<.05 are denoted with asterisk (*), indicating that both exercise groups of mice were different from the placebo group of mice that did not exercise (D).