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High osmolarity induces a shmoolike morphology in hog1 and pbs2 mutants. (Top row) Cells incubated in YEPD liquid medium; (middle row) cells treated with α-factor in YEPD for 2 hr; (bottom row) cells treated with 1 m sorbitol in YEPD for 6 hr. Strains SO329 (wild type), SO330 (hog1), SO382 (pbs2), SO353 (ssk1 sho1), SO143 (hog1 ste12), and SO356 (hog1 sho1) were grown to log phase and shifted into fresh liquid media as indicated.
