Figure 2.
Telomerase from developing macronuclei assembles into higher order complexes. S100 macronuclear lysates from E. crassus undergoing macronuclear development were fractionated by gel filtration chromatography as described in Fig. 1. Assays and molecular weight markers are as in Fig. 1. (Lane (1) Macronuclei; (lane 2) S100; (lanes 3–26) even-numbered fractions from the column elution profile. G3, 11, and 19 correspond to synthesis of the third, eleventh, and nineteenth dG, respectively, in the G4T4 repeated ladder. The peaks corresponding to distinctive telomerase complexes are indicated.