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. 1998 Sep 15;12(18):2921–2931. doi: 10.1101/gad.12.18.2921

Figure 3.

Figure 3

Figure 3

Distinct forms of telomerase are present in developing macronuclei. Individual telomerase complexes fractionated from developing macronuclear lysates were concentrated by dialysis into 40% PEG, rechromatographed by gel filtration and assayed for telomerase activity. Rechromatographed 550-kD (Fig. 2, fractions 16–18) (A) and 1600-kD (Fig. 2, fractions 12–14) (B) complexes are shown. (Lanes 1) Reactions with a macronuclear lysate; (lane 2) reactions with the isolated, concentrated telomerase peaks (see Materials and Methods) prior to rechromatography; (lanes 3–26) superose 6 column elution profile. Assays and molecular mass markers are as in Fig. 1.