Table 3.

Effects of RIM15 deletion

	RIM15/RIM15a		rim15Δ/rim15Δa
	log	stat	log	stat
Enzymes and metabolites
Tre6P synthase (μmole/sec per gram protein)	0.33	1.32	0.37	1.17
trehalase (μmole/sec per gram protein)	0.09	0.17	0.05	0.16
invertase (μmole/sec per gram protein)	2.00	12.93	1.93	12.06
SSA3–lacZ inductionb (Miller units)	4.30	105.20	2.40	16.30
trehalose (gram/gram protein)	<0.001	0.199	<0.001	0.009
glycogen (mg/gram protein)	1.63	38.53	0.93	11.36
Thermotolerancec (% survival)	0.13	35.60	0.07	0.03
Stationary phase survivald (%)		100.00		20.20
Budded cellse (%)	65.70	0.90	65.70	24.30

^aWild-type and rim15Δ/rim15Δ strains were YEF473 and AR2, respectively. All experiments were carried out on YPD (2% glucose) medium using either log phase (log) or 4-day-old stationary phase (stat) cells, except where otherwise stated. Values represent means of at least three independent experiments; s.d.s were, in each case, <10% of the corresponding means. 

^bβ-Galactosidase activities were measured to monitor the induction of an SSA3–lacZ fusion gene (from plasmid pWB204Δ–236). 

^cThermotolerance was measured as the percent survival following a heat shock for 8 min at 50°C (log-phase cells) or 20 min at 53°C (stationary-phase cells). 

^dThe percentage of viable cells was determined by the colony-forming efficiency on YPD agar of 10-day-old stationary-phase cultures. 

^eThe percentage of budded cells was determined by microscopic examination of at least 200 cells.