Table 4.

Suppression of bcy1-1 phenotypes by overexpression of RIM15

Strain	Relevant genotype	Plasmid	Trehalose (gram/gram protein)	SSA3–lacZ induction (Miller units)	Thermotolerance (% survival)
SP1	BCY1	YCpADH1	0.39 ± 0.05	137.8 ± 37.5	39.8 ± 3.7
SP1	BCY1	YCpADH1–RIM15	0.56 ± 0.11	299.9 ± 76.0	73.0 ± 2.4
T16-11A	bcy1-1	YCpADH1	0.12 ± 0.06	41.4 ± 11.1	12.6 ± 2.9
T16-11A	bcy1-1	YCpADH1–RIM15	0.24 ± 0.02	123.9 ± 25.7	43.0 ± 10.9

Cells were grown to stationary phase (5 days) on SD medium containing 1% glucose. Values represent means ± s.d.s of three to six independent experiments. β-Galactosidase activities were measured to monitor the induction of an SSA3–lacZ fusion gene (from plasmid pWB204Δ–236). Thermotolerance was measured as the survival following a heat shock for 20 min at 50°C.