Table 2.
PCR primers used to screen the RPSA
| Primer identity | Primer sequences | Nucleotide position | Product size |
|---|---|---|---|
| RPSA 1 | A: GAT GTG CGC TGT TCC GTA AT B: TCA TGT TCA TGA CCC AAC CC |
1026-1601 | 576 bp |
| RPSA 2 | A: GGA AAG AGT GGC AGA AAG CC B: TGC TGG GAT TAC AGG CGT AG |
1866-2431 | 566 bp |
| RPSA 3 | A: CCA GTG CCC AGA AGT GCT TA B: TTC ATC GGC CAG TCA GTA GC |
4252-4681 | 430 bp |
| RPSA 4 | A: GCT TGC TGT TTG GGT TTG AC B: TTC CTT ACC CCA CTC CCA AC |
5171-5799 | 629 bp |
| RPSA 5 | A: AAG CAA AAC TTG TCA GTC CCT G B: CAA CCA TTT TTC CAT GCT GC |
5421-5990 | 570 bp |
'A' indicates the forward primer, and 'B' indicates the reverse primer.