Figure 5.

Primary transcript in situ hybridization detecting human β-globin and mouse α-globin transcription. E13.5 fetal liver cells with the indicated transgene makeup (see also Fig. 4) were disrupted in PBS, spotted on polylysine-coated slides, fixed, and subjected to primary transcript in situ hybridization with two sets of oligonucleotide probes detecting mouse α-globin (green) and human β-globin (red) nuclear transcription signals. The probes for human β-globin also detect cytoplasmic mRNA; this is particularly evident in the mutant reporter/mEKLF and the control reporter cells. Representative examples of 20 cells are shown for each transgene combination.