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CaMKII phosphorylation of Cx43-CT at S314. (A) Extracted chromatogram of m/z 659.2613–659.2717. (B) MS spectrum of m/z 659.2708 represents triply charged peptide QASEQNWANYSAEQNR (one phosphorylation site). (C) CID MS2 spectrum indicates phosphorylation on S314. (D) ETD MS2 spectrum indicates phosphorylation on S314. See Supplemental Table S5 for complete list of theoretical (ProteinProspector) and observed fragment ions.
