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. 1999 Dec 1;13(23):3125–3135. doi: 10.1101/gad.13.23.3125

Figure 3.

Figure 3

Fyn tyrosine kinase is recruited to the caveolae-like domains upon ephrin-A5 engagement. (A) NIH-3T3 cells expressing ephrin-A5 were incubated for different times with EphA5–Fc, in the absence or presence of PP1 as indicated. Caveolae-like fractions (cav) were isolated and analyzed by Western blot using in parallel 4G10 and an antibody specific for Fyn. (B) NIH-3T3 cells expressing ephrin-A5 were fractionated on a sucrose gradient. Caveolae-like fractions (C) and soluble fractions (S) were tested by Western blot for the presence of Fyn and Src proteins. Molecular weight mass are indicated in kD.