Figure 5.

Fyn tyrosine kinase is essential for signaling in caveolae-like microdomains in vivo. (A) Caveolae-like microdomains (C) and detergent soluble fractions (S) were isolated from cortex of mice at different stages of development (E18 and P3). Samples were analyzed by Western blot using 4G10, caveolin-2, and Fyn-specific antibodies. (B) Caveolae-like fractions (cav) were isolated from brain of mice that were genotyped wild type (Fyn^+/+), defective for Fyn kinase (Fyn^−/−), or heterozygous (Fyn^+/−). Fractions were analyzed by Western blotting using 4G10 and an antibody specific for caveolin-2.